The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum.
Identifieur interne : 000D57 ( Main/Exploration ); précédent : 000D56; suivant : 000D58The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum.
Auteurs : Nikola Ptá Ková [République tchèque] ; Jitka Klempová [République tchèque] ; Michal Obo Il [République tchèque] ; Sylvie Ned Lová [République tchèque] ; Jan Lochman [République tchèque] ; Tomáš Kašparovsk [République tchèque]Source :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2015.
Descripteurs français
- KwdFr :
- Acides gras (métabolisme), Concentration en ions d'hydrogène (MeSH), Espace extracellulaire (MeSH), Espèces réactives de l'oxygène (métabolisme), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Maladies des plantes (microbiologie), Membrane cellulaire (métabolisme), Monoxyde d'azote (métabolisme), Mort cellulaire (MeSH), Peroxydation lipidique (MeSH), Phytophthora (pathogénicité), Phytophthora (physiologie), Protéines fongiques (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Stérols (métabolisme), Tabac (génétique), Tabac (microbiologie), Tabac (métabolisme), Transcription génétique (MeSH).
- MESH :
- génétique : Feuilles de plante, Protéines végétales, Tabac.
- microbiologie : Feuilles de plante, Maladies des plantes, Tabac.
- métabolisme : Acides gras, Espèces réactives de l'oxygène, Feuilles de plante, Membrane cellulaire, Monoxyde d'azote, Protéines fongiques, Protéines végétales, Stérols, Tabac.
- pathogénicité : Phytophthora.
- physiologie : Phytophthora.
- Concentration en ions d'hydrogène, Espace extracellulaire, Mort cellulaire, Peroxydation lipidique, Transcription génétique.
English descriptors
- KwdEn :
- Cell Death (MeSH), Cell Membrane (metabolism), Extracellular Space (MeSH), Fatty Acids (metabolism), Fungal Proteins (metabolism), Hydrogen-Ion Concentration (MeSH), Lipid Peroxidation (MeSH), Nitric Oxide (metabolism), Phytophthora (pathogenicity), Phytophthora (physiology), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Reactive Oxygen Species (metabolism), Sterols (metabolism), Tobacco (genetics), Tobacco (metabolism), Tobacco (microbiology), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Fatty Acids, Fungal Proteins, Nitric Oxide, Plant Proteins, Reactive Oxygen Species, Sterols.
- genetics : Plant Leaves, Tobacco.
- metabolism : Cell Membrane, Plant Leaves, Tobacco.
- microbiology : Plant Diseases, Plant Leaves, Tobacco.
- pathogenicity : Phytophthora.
- physiology : Phytophthora.
- Cell Death, Extracellular Space, Hydrogen-Ion Concentration, Lipid Peroxidation, Transcription, Genetic.
Abstract
Cryptogein, a protein from oomycete Phytophthora cryptogea, induces a hypersensitive cell death in Nicotiana tabacum. We prepared a new series of cryptogein mutant proteins with altered abilities to bind sterols and with altered charge distribution in the proteins. The effect of the mutations on the cryptogein ability to induce plant defence mechanisms associated with hypersensitive cell death were examined. Our results with new mutants support the previous findings that the sterol binding does not influence synthesis of ROS, cytosol acidification and development of leaf necrosis as these events seem to be more likely affected by the charge distribution and the overall protein structure. This hypothesis was also applicable on other mechanisms involved in the execution of plant cell death such as the NO generation, the stimulation of lipid peroxidation (determination of malondialdehyde and hydroxy fatty acids levels) and LOX gene transcription. In addition, the ability to bind sterols was found to serve not only for pathogen utilisation in its own metabolism but also to have an important function for the destabilization of plant membrane facilitating the pathogen spread inside the plant tissue as well as intensively contributing to the development of plant cell death. Considering the insertion of charged amino acid residues in the protein structure, the change localized in the protein surface affected its biological activity more effectively than that change inside the protein cavity. Moreover, the insertion of negative charged amino acids influenced mainly the events involved in the early phase of defence reaction, while the positive residues affected especially the necrotic activity of cryptogein.
DOI: 10.1016/j.plaphy.2015.09.009
PubMed: 26433637
Affiliations:
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We prepared a new series of cryptogein mutant proteins with altered abilities to bind sterols and with altered charge distribution in the proteins. The effect of the mutations on the cryptogein ability to induce plant defence mechanisms associated with hypersensitive cell death were examined. Our results with new mutants support the previous findings that the sterol binding does not influence synthesis of ROS, cytosol acidification and development of leaf necrosis as these events seem to be more likely affected by the charge distribution and the overall protein structure. This hypothesis was also applicable on other mechanisms involved in the execution of plant cell death such as the NO generation, the stimulation of lipid peroxidation (determination of malondialdehyde and hydroxy fatty acids levels) and LOX gene transcription. In addition, the ability to bind sterols was found to serve not only for pathogen utilisation in its own metabolism but also to have an important function for the destabilization of plant membrane facilitating the pathogen spread inside the plant tissue as well as intensively contributing to the development of plant cell death. Considering the insertion of charged amino acid residues in the protein structure, the change localized in the protein surface affected its biological activity more effectively than that change inside the protein cavity. Moreover, the insertion of negative charged amino acids influenced mainly the events involved in the early phase of defence reaction, while the positive residues affected especially the necrotic activity of cryptogein. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26433637</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>97</Volume><PubDate><Year>2015</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum.</ArticleTitle><Pagination><MedlinePgn>82-95</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2015.09.009</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(15)30111-X</ELocationID><Abstract><AbstractText>Cryptogein, a protein from oomycete Phytophthora cryptogea, induces a hypersensitive cell death in Nicotiana tabacum. We prepared a new series of cryptogein mutant proteins with altered abilities to bind sterols and with altered charge distribution in the proteins. The effect of the mutations on the cryptogein ability to induce plant defence mechanisms associated with hypersensitive cell death were examined. Our results with new mutants support the previous findings that the sterol binding does not influence synthesis of ROS, cytosol acidification and development of leaf necrosis as these events seem to be more likely affected by the charge distribution and the overall protein structure. This hypothesis was also applicable on other mechanisms involved in the execution of plant cell death such as the NO generation, the stimulation of lipid peroxidation (determination of malondialdehyde and hydroxy fatty acids levels) and LOX gene transcription. In addition, the ability to bind sterols was found to serve not only for pathogen utilisation in its own metabolism but also to have an important function for the destabilization of plant membrane facilitating the pathogen spread inside the plant tissue as well as intensively contributing to the development of plant cell death. Considering the insertion of charged amino acid residues in the protein structure, the change localized in the protein surface affected its biological activity more effectively than that change inside the protein cavity. Moreover, the insertion of negative charged amino acids influenced mainly the events involved in the early phase of defence reaction, while the positive residues affected especially the necrotic activity of cryptogein. </AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ptáčková</LastName><ForeName>Nikola</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Klempová</LastName><ForeName>Jitka</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Obořil</LastName><ForeName>Michal</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nedělová</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lochman</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kašparovský</LastName><ForeName>Tomáš</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Masaryk University, Faculty of Science, Department of Biochemistry, Kotlářská 2, 611 37, Brno, Czech Republic. Electronic address: tkasp@sci.muni.cz.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>09</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005227">Fatty Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013261">Sterols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C061032">cryptogein protein, Phytophthora cryptogea</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005110" MajorTopicYN="N">Extracellular Space</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005227" MajorTopicYN="N">Fatty Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015227" MajorTopicYN="N">Lipid Peroxidation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013261" MajorTopicYN="N">Sterols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cryptogein</Keyword><Keyword MajorTopicYN="N">Hydroxy fatty acids analysis</Keyword><Keyword MajorTopicYN="N">Hypersensitive response</Keyword><Keyword MajorTopicYN="N">Lipid peroxidation</Keyword><Keyword MajorTopicYN="N">Mutant proteins</Keyword><Keyword MajorTopicYN="N">Nicotiana tabaccum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>05</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26433637</ArticleId><ArticleId IdType="pii">S0981-9428(15)30111-X</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2015.09.009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country><region><li>Moravie</li></region><settlement><li>Brno</li></settlement></list><tree><country name="République tchèque"><region name="Moravie"><name sortKey="Pta Kova, Nikola" sort="Pta Kova, Nikola" uniqKey="Pta Kova N" first="Nikola" last="Ptá Ková">Nikola Ptá Ková</name></region><name sortKey="Kasparovsk, Tomas" sort="Kasparovsk, Tomas" uniqKey="Kasparovsk T" first="Tomáš" last="Kašparovsk">Tomáš Kašparovsk</name><name sortKey="Klempova, Jitka" sort="Klempova, Jitka" uniqKey="Klempova J" first="Jitka" last="Klempová">Jitka Klempová</name><name sortKey="Lochman, Jan" sort="Lochman, Jan" uniqKey="Lochman J" first="Jan" last="Lochman">Jan Lochman</name><name sortKey="Ned Lova, Sylvie" sort="Ned Lova, Sylvie" uniqKey="Ned Lova S" first="Sylvie" last="Ned Lová">Sylvie Ned Lová</name><name sortKey="Obo Il, Michal" sort="Obo Il, Michal" uniqKey="Obo Il M" first="Michal" last="Obo Il">Michal Obo Il</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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